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Engineering one cyanobacterium at a time

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The major topics currently under investigation in our laboratory are

Research Tools

We use the following tools for our laboratory work:

  • modern molecular cloning methods (BioBrick assembly, Gibson & Aqua Cloning)
  • report-gene activity measurements
  • RNA analysis (purification, hybridization, qPCR, in-line probing)
  • RNA interaction with RNA, proteins, ligands
  • recombinant protein expression in E. coli and cyanobacteria
  • protein purification and characterization (interaction studies, activity assays)
  • metabolic engineering of cyanobacteria
  • phenotypic analysis of cyanobacterial mutants

We use the following tools for our computational work:

  • In silico cloning with SnapGene
  • Data analyses with Python 3 and Jupyter Notebook/Lab
  • Data analyses with R and RStudio
  • Comparative genomic analysis
  • Flux Balance Analysis with COBRApy
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